“Reproduction bar” embryo fragment still can transplant?
The first Affiliated Hospital of Guangzhou University of Chinese Medicine wechat subscription column, every Monday push!The column articles are written by reproductive medicine experts in the hospital.Content covers all aspects of reproductive medicine knowledge, practical, wonderful!Stay tuned!Can an embryo be transplanted if it has fragments?Embryo fragmentation is a common phenomenon during embryo development in vitro.The embryo lab scores the fragments based on their number, size and distribution.Fragment score is also one of the important factors in embryo quality grading.Why are fragments of embryos created?Studies have shown that there is a certain relationship between the production of embryo fragments and apoptosis.When an embryo is damaged by its own genetics or by external environmental stress, it activates the self-protective mechanism of DNA repair, excreting harmful components and resulting in fragmentation.A small amount of debris can dissolve or be absorbed by itself during culture without affecting embryonic viability.However, when there are too many fragments, DNA damage cells can be removed by apoptotic mechanism in the early stage of embryo development, which eventually leads to embryo degeneration and avoids passing genetic damage to offspring.Comparison of embryos with different fragmentation rates The factors affecting embryo fragmentation are mainly divided into the following aspects.1. Maternal factors.Some pathological factors such as hydrosalpinx, inflammatory effusion, endometriosis and the presence of various negative factors in endometrium may lead to embryo fragmentation.2. Egg and sperm quality.Poor egg and sperm quality is a congenital condition for embryo fragmentation.Different female patients have differences in the function and quality of ovum due to genetic differences in ovum.In addition, when sperm DNA damage is severe in men, exceeding the DNA repair ability of eggs and embryos, fragments may also occur.3. Clinical ovulation induction factors.Controlled ovulation induction is a method to control ovulation induction by using exogenous gonadotropin in assisted reproductive technology.Exogenous gonadotropins may affect cytoplasmic and nuclear capacities, but it is not clear whether specific regimenes lead to higher or lower fragmentation rates.4. Human assisted reproduction laboratory — factors of embryo culture environment.The undesirable culture environment in vitro is the epigenetic factor of embryo fragmentation, which will bring pressure to the early development of embryo.When the laboratory embryo culture environment is not good, such as indoor temperature, humidity, accuracy and cleanliness of equipment consumables, and skilled operation of technicians, it will lead to the generation of embryo fragments, and ultimately affect the development potential of embryos.Therefore, embryo laboratory should regularly test the culture environment, do a good job in quality management, reduce the influence of culture conditions on embryo development.Embryo fragments used in embryo culture is an important index to evaluate embryo quality and predict pregnancy outcome.Too many embryo fragments can affect the tight connection of embryo blastocyst and blastocyst formation, and affect embryo implantation rate.Therefore, adverse factors should be reduced as far as possible to improve the pregnancy rate of assisted reproductive technology.The Department of Reproductive Medicine of the First Affiliated Hospital of Guangzhou University of Chinese Medicine is a comprehensive department integrating medical treatment, scientific research and teaching. It is also the only two human assisted reproductive technology institutions with the characteristics of traditional Chinese medicine hospitals in Guangdong Province.The department is committed to the treatment of reproductive disorders with the combination of Traditional Chinese and Western medicine, combined with modern assisted reproductive technology on the basis of traditional Chinese medicine, for infertility patients to provide personalized diagnosis and treatment.The Reproductive Medicine Department of our hospital was established in October 2010, covering an area of more than 1600m2. It is mainly located at the reproductive medicine clinic on the 5th, 6th and 7th floors of Building 7 and the 3rd and 5th floors of the outpatient building.Artificial insemination technology (AIH) has been officially carried out for many years, and the pregnancy rate of AIH is 15.5% at present, ranking the top of AIH pregnancy rate among major hospitals in Guangdong Province.After years of unremitting efforts, the department has obtained the formal operation qualification of in vitro fertilization-embryo transfer (IVF-ET, commonly known as test-tube baby technology) and its derivative technology through verification. At present, the department is in the stage of vigorous development. In the past six months, the pregnancy rate of embryo frozen transfer cycle is 70.83%, which has reached the leading level of peers in the province.This subject from guangzhou university of Chinese medicine combine traditional Chinese and western medicine for reproductive medicine discipline leaders, domestic well-known professor male reproductive experts weeks little tiger as a leader, technical experience with rich clinical, laboratory and nursing team, clinical environment comfortable warmth, dedicated to provide convenient and effective for each pair of infertile couples, professional and personalized service.The department opened male infertility clinic, female infertility clinic, fertility assessment and traditional Chinese medicine characteristics of the clinic, play to the traditional Chinese medicine “peiyuan spermatogenesis, menstruation seeds, pregnancy after abortion” advantage, effectively improve the success rate of infertility patients, won a good reputation.Service items of reproductive medicine department ● Artificial insemination technology (AIH);● In vitro fertilization – embryo transfer (IVF-ET, commonly known as the first generation of test-tube baby technology);● Intracytoplasmic sperm microinjection technology (ICSI, commonly known as the second generation of ivf technology);● Monitoring ovulation by transvaginal ultrasound;● Medication to stimulate ovulation;● Painless anesthesia egg extraction;● Testicular puncture sperm extraction (TESE);● Sperm freezing;● Embryo freezing and thawing technology;● Embryo assisted incubation;● Fertility assessment and TCM recuperation clinic.Mei Junyan, embryologist, Department of Reproductive Medicine, The First Affiliated Hospital of Guangzhou University of Chinese Medicine.Master’s degree: Embryo engineering and transgenic pharmaceutical;Since 2014, various embryo and microscopic operations, including nuclear transfer and microinjection, have been started.He participated in the study of oocyte maturation and nuclear transfer in vitro.Currently, I am mainly responsible for laboratory quality control, familiar with and mastering various embryo operations including egg picking, fertilization, assisted hatching, embryo freezing and resuscitation, and participated in the laboratory approval work of reproductive medicine.Medical treatment: artificial insemination and ivf baby semen processing and various embryo operations.Editor: Liu Qingjun review: Zheng Xiaoying, Yang Yan Responsibility: Zhong Tiansend